程序代码：

Public Class Form1
    '窗体上随便画一个datagridview控件
    Private Sub Form1_Load(ByVal sender As System.Object, ByVal e As System.EventArgs) Handles MyBase.Load
        DataGridView1.ColumnCount = 3
        DataGridView1.Rows.Add()
        DataGridView1.AllowUserToAddRows = False '设置成不能自动添加行
    End Sub

    Private Sub DataGridView1_KeyUp(ByVal sender As Object, ByVal e As System.Windows.Forms.KeyEventArgs) Handles DataGridView1.KeyUp
        If DataGridView1.CurrentRow.Index = DataGridView1.Rows.Count - 1 And DataGridView1.CurrentCell.ColumnIndex = DataGridView1.ColumnCount - 1 Then
            DataGridView1.Rows.Add()
        End If
    End Sub

End Class