import wx
import time

class MyFrame(wx.Frame):
    def __init__(self,parent,id):
        wx.Frame.__init__(self,parent,id,'秒倒计时',size=(300,160))#设定窗体
        self.seconds=0
        panel=wx.Panel(self)#设定板面
        self.tim=wx.StaticText(panel,label='0',size=(300,50),style=wx.ALIGN_CENTRE)
        self.tim.SetBackgroundColour('black') #设置静态文本的背景颜色
        self.tim.SetForegroundColour('green') #设置静态文本的前景色
        font=wx.Font(40,wx.DECORATIVE,wx.DEFAULT,wx.NORMAL) #设定文本的字体
        self.tim.SetFont(font)
        self.st=wx.StaticText(panel,label='时间设定（秒）：')
        self.st1=wx.TextCtrl(panel,style=wx.TE_PROCESS_ENTER)
        self.st1.Bind(wx.EVT_TEXT_ENTER,self.enter)#文本框的事件
        self.bott=wx.Button(panel,label='开始')
        self.bott1=wx.Button(panel,label='暂停')
        self.bott.Bind(wx.EVT_BUTTON,self.Nostart)
        self.bott1.Bind(wx.EVT_BUTTON, self.Nostop)
        self.timer=wx.Timer(self)#设定定时器
        self.timer.Bind(wx.EVT_TIMER,self.OnTimer)#绑定定时器
        hsize = wx.BoxSizer(wx.HORIZONTAL) #横向版面布局
        hsize.Add(self.st, proportion=0, flag=wx.ALL, border=5)
        hsize.Add(self.st1, proportion=0, flag=wx.ALL, border=5)
        hsize1 = wx.BoxSizer(wx.HORIZONTAL)
        hsize1.Add(self.bott, proportion=0, flag=wx.ALIGN_CENTRE, border=5)
        hsize1.Add(self.bott1, proportion=0, flag=wx.ALIGN_CENTRE, border=5)
        vsize = wx.BoxSizer(wx.VERTICAL)#纵向版面布局
        vsize.Add(self.tim, proportion=0, flag=wx.EXPAND, border=0)
        vsize.Add(hsize, proportion=0, flag=wx.EXPAND, border=5)
        vsize.Add(hsize1, proportion=0, flag=wx.ALIGN_CENTRE, border=5)
        panel.SetSizer(vsize)#启动板面布局

    def enter(self,event):#文本框事件
        self.seconds=int(self.st1.GetValue())*1000
        mm=int((self.seconds/1000)//60)
        ss=int((self.seconds/1000)%60)
        xx=int((self.seconds/1000)%10)
        text_time='{:02d}:{:02d}.{:02d}'.format(mm,ss,xx)
        self.tim.SetLabel(text_time)


    def OnTimer(self,event): 定时器事件
        self.seconds-=1
        mm = int((self.seconds / 1000) // 60)
        ss = int((self.seconds / 1000) % 60)
        xx = int((self.seconds / 1000) % 10)
        text_time = '{:02d}:{:02d}.{:02d}'.format(mm, ss, xx)
        self.tim.SetLabel(text_time)



    def Nostart(self,event):
        self.timer.Start(1000)#每1秒启动一次定时器
    def Nostop(self,event):
        self.timer.Stop()#停止定时器




if __name__ == '__main__':
    app=wx.App()
    frame = MyFrame(parent=None, id=-1)

    frame.Show()
    app.MainLoop()

谢谢，确实是我写错了，太感谢了。但还是不能运行下去，self.timer.Stop()不能停止。