private void button1_Click(object sender, EventArgs e)
        {

            string Con = "provider=MIcrosoft.ACE.OLEDB.12.0;" + @"Data source=D:\c\DataBase\student.accdb";//第二个参数为文件的路径  
            OleDbConnection conn = new OleDbConnection(Con);
            conn.Open();//建立连接
            string no = "'" + textBox1.Text + "'", name = "'" + textBox2.Text + "'",a= "'" + textBox3.Text + "'",
            tal = "'" + textBox4.Text + "'",gen = "'" + comboBox1.Text + "'";
            string insert = "INSERT INTO student(studentno,studentname,age,tall,gender)values(n,name,a,tal,gen)";
            OleDbCommand mycommand = new OleDbCommand(insert, conn);
            mycommand.ExecuteNonQuery();           //这里提示至少有一个值没有指定值，请问是哪里的问题！
            OleDbDataAdapter inst = new OleDbDataAdapter("SELECT * FROM student", conn);//选择全部内容

            DataSet ds = new DataSet();//临时存储
            inst.Fill(ds);//用inst填充ds
            dataGridView1.DataSource = ds.Tables[0];//展示ds第一张表到dataGridView1控件
            conn.Close();
        }

你得用字符串拼接的方式 把变量的值放到同一个字符串里面
而不是在字符串里面写变量 n,name,a,tal,gen
那只是字符串的字符 不会扩展填充成前面的字符串

能简单举例吗？麻烦了

string insert = "INSERT INTO student(studentno,studentname,age,tall,gender)values(n,name,a,tal,gen)";
你这里的no,name,a,tal,gen不是变量，他们是字符串的一部分


string insert = "INSERT INTO student(studentno,studentname,age,tall,gender)values(" + no + "," + name + "," + a + "," + tal + "," + gen + ")";

谢谢，非常感谢！可以了！！